Table 3 Primers used in this study
PrimersSequence (5′-3′)Restriction site
MO608AATTCATATGCAAGGGAAAATGATGAAAAANdeI
MO612GTAGGATCCAATTTCAATCTCGTACCAAAGGTATBamHI
Cjej_9CATCACAGCAGCGGC
Cjej_10GCAGCCGGATCCTAT
D44A_1GGTgcaGCTGTGCTTGIntroduction of D44A mutation and PvuII site
D44A_2CAAGCACAGCtgcACC
E116Q_1CAGAcGCGTTAGAtGCCATIntroduction of E116Q mutation and MluI site
E116Q_2ATGGCaTCTAACGCgTCTG
E116D_1CAGAcGCGTTAcAAGCCATIntroduction of E116D mutation and MluI site
E116D_2ATGGCTTgTAACGCgTCTG