Table 2 Kinetic parameters of interfacial mutants and active site mutants of pig kidney FBPase
EnzymeVmax (µM/min)Km (µM)kcat (s−1)CE (kcat/Km )AMP Hill coefficient
Y164A27.55.30 (± 0.6)13.5 (± 1.2)2.602.8 (± 0.2)
M177A_126.14.20 (± 0.6)12.8 (± 1.3)3.102.6 (± 0.2)
M177A_2*23.44.80 (± 0.5)11.5 (± 1.2)2.402.8 (± 0.1)
K112A54.73.25 (± 0.2)26.8 (± 2.5)8.251.0 (± 0.1)
Y113A55.52.50 (± 0.3)27.2 (± 2.6)10.91.2 (± 0.1)
M248D62.21.25 (± 0.4)30.5 (± 2.9)24.42.5 (± 0.2)
L56A52.35.20 (± 0.2)25.6 (± 1.9)4.902.3 (± 0.2)
L73A50.55.10 (± 0.3)22.5 (± 2.8)4.412.1 (± 0.2)
WT41.84.80 (± 0.5)20.5 (± 1.2)4.302.2 (± 0.1)
  • * Second clone tested.