Table 2 Components composition of lysis buffer and organic reagents used in SDS-based DNA extraction
Components composition of SDS lysis buffer (pH 8.0)
NumberSDS (w/v)NaCl (mM)Tris/HCl (mM)EDTA (mM)
10.5%25010025
22%25010025
35%25010025
42%010025
52%15010025
62%25010025
72%50010025
82%2501025
92%2505025
102%25010025
112%25020025
122%2501005
132%25010025
142%25010050
152%250100100
Organic reagents used to extract and precipitate DNA
ProcedureFirst extractionThird extractionFirst precipitationSecond precipitation
AP: C: IC: IEthanolEthanol
BC: IC: IEthanolEthanol
CP: C: I/EthanolEethanol
DP: C: IC: IKAc + 95% EthanolEthanol
EP: C: IC: IIsopropanolEthanol
FC: IC: IEthanolIsopropanol
GC: IC: IIsopropanolIsopropanol
HC: IC: IIsopropanolEthanol