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Accepted Manuscript

Metabolomic Analysis Reveals a Protective Effect of Fu-Fang-Jin-Qian-Chao Herbal Granules on Oxalate-Induced Kidney Injury

Wei Chen, Wen-Rui Rui Liu, Jie-Bin Bin Hou, Jia-Rong Rong Ding, Zhong-Jiang Jiang Peng, Song-Yan Yan Gao, Xin Dong, Jun-Hua Hua Ma, Qi-Shan Shan Lin, Jian-Rao Rao Lu, Zhi-Yong Yong Guo
Bioscience Reports Feb 08, 2019, BSR20181833; DOI: 10.1042/BSR20181833
Wei Chen
Changhai Hospital, Second Military Medical University, Shanghai, China
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Wen-Rui Rui Liu
Changhai Hospital, Second Military Medical University, Shanghai, China
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Jie-Bin Bin Hou
Department of Geriatric Nephrology, Chinese PLA General Hospital, Beijing, China
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Jia-Rong Rong Ding
Changhai Hospital, Second Military Medical University, Shanghai, 200433, China
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Zhong-Jiang Jiang Peng
Changhai Hospital, Second Military Medical University, Shanghai, 200433, China
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Song-Yan Yan Gao
School of Pharmacy, Second Military Medical University, Shanghai, China
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Xin Dong
School of Pharmacy, Second Military Medical University, Shanghai, China
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Jun-Hua Hua Ma
Guangxi Wantong Pharmaceutical Co., Ltd, Guangxi, China
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Qi-Shan Shan Lin
Proteomics/Mass Spec Facility, Center for Functional Genomics, University at Albany, Rensselaer, NY, New York, United States
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Jian-Rao Rao Lu
Department of Nephrology, Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
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Zhi-Yong Yong Guo
Changhai Hospital, Second Military Medical University, Shanghai, 200433, China
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  • For correspondence: drguozhiyong@163.com
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Abstract

Nephrolithiasis is one of the world's major public health burdens with a high incidence and a risk of persistent renal dysfunction. Fu-Fang-Jin-Qian-Chao granules (FFJQC), a traditional Chinese herb formula, is commonly used in treatment of nephrolithiasis. However, the therapeutic mechanism of FFJQC on kidney stone has still been a mystery. The objective of this study is to explore the therapeutic mechanism of FFJQC on kidney injury and identify unique metabolomics patterns using a mouse model of kidney stone induced by a calcium oxalate deposition. Von Kossa staining and immuno-histopathological staining of osteopontin (OPN), cluster of differentiation 44 (CD44) and calbindin-D28k were conducted on renal sections. Biochemical analysis was performed on serum, urine, and kidney tissues. A metabolomics approach based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used for serum metabolic profiling. The immunohistopathological and biochemical analysis showed the therapeutic benefits of FFJQC. The expression levels of OPN and CD44 were decreased while calbindin-D28k increased after the CaOx injured mice were treated with FFJQC. In addition, total of 81 serum metabolites were identified to be associated with protective effects of FFJQC on CaOx crystal injured mice. Most of these metabolites were involved in purine, amino acid, membrane lipid and energy metabolism. Potential metabolite biomarkers were found for CaOx crystal-induced renal damage. Potential metabolite biomarkers of CaOx crystal-induced renal damage were found. FFJQC shows therapeutic benefits on CaOx crystal injured mice via regulation of multiple metabolic pathways including amino acids, purine, pyrimidine, glycerolipid, arachidonic acid, ect.

  • Fu-Fang-Jin-Qian-Chao
  • mass spectrometry
  • metabolomics
  • Oxalate crystal
  • Kidney stone
  • ©2019 The Author(s)

This is an Accepted Manuscript; not the final Version of Record. You are encouraged to use the final Version of Record that, when published, will replace this manuscript and be freely available under a Creative Commons licence.

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Metabolomic Analysis Reveals a Protective Effect of Fu-Fang-Jin-Qian-Chao Herbal Granules on Oxalate-Induced Kidney Injury
Wei Chen, Wen-Rui Rui Liu, Jie-Bin Bin Hou, Jia-Rong Rong Ding, Zhong-Jiang Jiang Peng, Song-Yan Yan Gao, Xin Dong, Jun-Hua Hua Ma, Qi-Shan Shan Lin, Jian-Rao Rao Lu, Zhi-Yong Yong Guo
Bioscience Reports Feb 2019, BSR20181833; DOI: 10.1042/BSR20181833
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Metabolomic Analysis Reveals a Protective Effect of Fu-Fang-Jin-Qian-Chao Herbal Granules on Oxalate-Induced Kidney Injury
Wei Chen, Wen-Rui Rui Liu, Jie-Bin Bin Hou, Jia-Rong Rong Ding, Zhong-Jiang Jiang Peng, Song-Yan Yan Gao, Xin Dong, Jun-Hua Hua Ma, Qi-Shan Shan Lin, Jian-Rao Rao Lu, Zhi-Yong Yong Guo
Bioscience Reports Feb 2019, BSR20181833; DOI: 10.1042/BSR20181833

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Keywords

Fu-Fang-Jin-Qian-Chao
mass spectrometry
metabolomics
Oxalate crystal
Kidney stone

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