Objective: MicroRNAs (miRNAs) are a class of non-coding RNAs that exert critical roles in various biological processes. The target of the present study was to identify the functional roles of microRNA-802 (miR-802) in regulating epithelial-mesenchymal transition (EMT) in prostate cancer (PCa).
Methods: miR-802 expression was investigated in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry. Transwell assay was conducted to investigate cell migration and invasion. Expression analysis of a set of EMT markers was performed to explore whether miR-802 is involved in EMT program. Xenograft model was established to investigate the function of miR-802 in carcinogenesis in vivo . The direct regulation of Flotillin-2 (Flot2) by miR-802 was identified using luciferase reporter assay.
Results: miR-802 was remarkably down-regulated in PCa tissues and cell lines. Gain-of-function trails showed that miR-802 serves as an "oncosuppressor" in PCa through inhibiting cell proliferation and promoting cell apoptosis in vitro . Overexpression of miR-802 significantly suppressed in vivo PCa tumor growth. Luciferase reporter analysis identified Flot2 as a direct target of miR-802 in PCa cells. Overexpressed miR-802 significantly suppressed EMT, migration and invasion in PCa cells by regulating Flot2.
Conclusions: We identified miR-802 as a novel tumor suppressor in PCa progression and elucidated a novel mechanism of the miR-802/Flot2 axis in the regulation of EMT, which may be a potential therapeutic target.
- prostate cancer
- epithelial-mesenchymal transition
- ©2017 The Author(s)
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