Extracorporeal pulsed electromagnetic field (PEMF) has shown the ability to regenerate tissue by promoting cell proliferation. Here, we investigated for the first time whether PEMF treatment could improve the myocardial ischemia/reperfusion (I/R) injury and uncovered its underlying mechanisms. In our study, we demonstrated for the first time that extracorporeal pulsed electromagnetic field(PEMF)has a novel effect on myocardial ischemia/reperfusion（I/R）injury. The number and function of circulating endothelial progenitor cells were increased in PEMF treating rats. The in-vivo results showed that per-treatment of PEMF could significantly improve the cardiac function in I/R injury group. In addition, PEMF treatment also reduced the apoptosis of myocardial cells by up-regulating the expression of anti-apoptosis protein(Bcl-2) and down-regulating the expression of pro-apoptosis protein(Bax). In vitro, the results showed that PEMF treatment could significantly reduce the apoptosis and ROS levels in primary neonatal rat cardiac ventricular myocytes(NRCMs) induced by hypoxia/reoxygenation(H/R). In particular, PEMF increased the phosphorylation of Akt and endothelial nitric oxide synthase(eNOS), which might be closely related to attenuated cell apoptosis by increasing the releasing of nitric oxide(NO). Therefore, our data indicated that PEMF could be a potential candidate for I/R injury.
- I/R injury
- ©2016 The Author(s)
This is an Accepted Manuscript; not the final Version of Record. You are encouraged to use the final Version of Record that, when published, will replace this manuscript and be freely available under a Creative Commons licence.