G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumor suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In this study, wild-type p53 binding to G-quadruplex formed by MYC promoter NHE III1 region was investigated. Wild-type p53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence. Apart from the full-length wild-type p53, its isolated C-terminal region (aa 320 - 393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wild-type p53 binds to MYC promoter region containing putative G-quadruplex motif in two wild-type p53-expressing cell lines. The results suggest that wild-type p53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.
- p53 protein
- DNA-protein interaction
- ©2016 The Author(s)
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