Eighty healthy male Wistar rats, aged 5 weeks, weighing 100-120 g, were utilized for establishing tumor-bearing models by immediate Walker-256 cancerous ascites injection and randomly divided to four groups (n=20) treated with 0.2 ml solution containing saline, 32P-colloid (0.3 mCi), endostatin gene (20 µg), endostatin gene combined with colloid 32P. The effect of endostatin combined with a small dose of 32P-colloidal on tumor growth<em> in vivo</em> was evaluated and the potential mechanism underlying the combined therapy was explored. We found that 32P-colloid combined with endostatin exhibited higher inhibitory effect upon tumor growth compared with application of 32P-colloid or endostatin alone, although three therapies all significantly inhibited tumor growth compared with saline control group. The higher inhibitory effect of 32P-colloid combined with endostatin upon tumor growth might be attributed to a synergistic effect of inhibiting angiogenesis by endostatin and inducing apoptosis by 32P-colloid, as demonstrated by microvessel density (MVD) and apoptotic index (AI) measurement. Combined therapy of 32P-colloid and endostatin probably serves as a novel and efficacious therapy of tumor growth.
- gene therapy
- ©2016 The Author(s)
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