Objective: To investigate adenovirus-mediated STAT3 infection and cisplatin (DDP) used alone or in combination on the growth of human Wilms tumor SK-NEP-1 cell subcutaneous xenografts in nude mice and the possible mechanisms. Methods: The human Wilms tumor cell line SK-NEP-1 were subcutaneously transplanted to established BALB/c nude mice xenograft model. Mice were randomly divided into five groups: blank control group, adenovirus control group (NC group), STAT3 group, CDDP group and STAT3 plus CDDP group (combination group). Tumor volume and tumor weight were observed during the therapeutic process. The expression levels of STAT3, GRP78 and BAX mRNAs and proteins were evaluated by HE staining and immunohistochemical analysis. Results: Compared with the STAT3 group or CDDP group, the tumor weight and volume was significantly reduced in the combination group (P <0.05). However, no stastic significance was found in NC group compared with the blank control group (P >0.05). HE staining and immunohistochemical analysis showed that STAT3, GRP78 and BAX protein expressions in the combination group was significantly higher than those in STAT3 group and CDDP group (P <0.05). Conclusion: Exogenous STAT3 and CDDP synergisticly may inhibit xenograft tumor growth through up-regulation of BAX protein via GRP78.
- Wilms tumor
- SK-NEP-1 cell
- ©2016 The Author(s)
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