Skip to main content

Main menu

  • Home
  • About the Journal
    • General Information
    • Scope
    • Editorial Board
    • Benefits of Publishing
    • Impact & Metrics
    • Advertising/Sponsorship
    • About the Biochemical Society
  • Current Issue
  • For Authors
    • Submit Your Paper
    • Submission Guidelines
    • Editorial Policy
    • Open Access Policy
    • Rights and Permissions
    • Biochemical Society Member Benefits
  • For Librarians
    • Open Access Policy
    • Terms and Conditions
      • Biochemical Journal- Terms and Conditions of Usage
      • Bioscience Reports- Terms and Conditions of Usage
  • For Readers
    • Rights and Permissions
    • Biochemical Society Member Benefits
  • Collections
  • Help
    • Technical Support
    • Contact Us
  • Other Publications
    • Biochemical Journal
    • Clinical Science
    • Bioscience Reports
    • Neuronal Signaling
    • Biochemical Society Transactions
    • Essays in Biochemistry
    • Emerging Topics in Life Sciences
    • Biochemical Society Symposia
    • Cell Signalling Biology
    • Glossary of Biochemistry and Molecular Biology
    • The Biochemist
    • Biochemical Society

User menu

  • Log-in
  • Subscribe
  • Contact Us

Search

  • Advanced search
  • Other Publications
    • Biochemical Journal
    • Clinical Science
    • Bioscience Reports
    • Neuronal Signaling
    • Biochemical Society Transactions
    • Essays in Biochemistry
    • Emerging Topics in Life Sciences
    • Biochemical Society Symposia
    • Cell Signalling Biology
    • Glossary of Biochemistry and Molecular Biology
    • The Biochemist
    • Biochemical Society

Log-in

Sign-up for alerts  
  • My Cart
Bioscience Reports
Browse Archive
Advanced Search
  • Home
  • About the Journal
    • General Information
    • Scope
    • Editorial Board
    • Benefits of Publishing
    • Impact & Metrics
    • Advertising/Sponsorship
    • About the Biochemical Society
  • Current Issue
  • For Authors
    • Submit Your Paper
    • Submission Guidelines
    • Editorial Policy
    • Open Access Policy
    • Rights and Permissions
    • Biochemical Society Member Benefits
  • For Librarians
    • Open Access Policy
    • Terms and Conditions
  • For Readers
    • Rights and Permissions
    • Biochemical Society Member Benefits
  • Collections
  • Help
    • Technical Support
    • Contact Us

Research Article

Endoplasmic reticulum stress is involved in spiral ganglion neuron apoptosis following chronic kanamycin-induced deafness

Yaqin Tu, Guorun Fan, Haiying Sun, Xiong Cai, Wen Kong
Bioscience Reports Feb 08, 2019, 39 (2) BSR20181749; DOI: 10.1042/BSR20181749
Yaqin Tu
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
Guorun Fan
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
Haiying Sun
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
Xiong Cai
Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: wenly-kong@163.comcaixiong@live.com
Wen Kong
Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
  • http://orcid.org/0000-0002-9275-8809
  • For correspondence: wenly-kong@163.comcaixiong@live.com
  • Article
  • Figures
  • Info & Metrics
  • Supplementary Data
  • PDF
Loading

Abstract

Aminoglycoside antibiotics-induced hearing loss is a common sensorineural impairment. Spiral ganglion neurons (SGNs) are first-order neurons of the auditory pathway and are critical for the maintenance of normal hearing. In the present study, we investigated the time-course of morphological changes and the degeneration process of spiral ganglion cells (SGCs) following chronic kanamycin-induced deafness and determined whether the endoplasmic reticulum (ER) stress was involved in the degeneration of SGNs. We detected density changes in SGCs and the expressions of Bip, inositol requirement 1 (IRE1)α, activating transcription factor-6α, p-PERK, p-eIF2α, CHOP, and caspase-12 at each time point after kanamycin treatment. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was also performed. The number of SGC deletions reached ∼50% at the 70th day after kanamycin administration and the ER of most SGCs were dilated. The expression of p-PERK, p-eIF2α, p-IRE1α, Bip, caspase-12, and Chop was significantly unregulated after kanamycin treatment. The number of SGCs that were positive for both TUNEL and caspase-12 increased from day 7 to 28. Taken together, these data demonstrate that ER stress was involved in kanamycin-induced apoptosis of SGNs. Kanamycin-induced SGN apoptosis is mediated, at least in part, by ER stress-induced upregulation of CHOP and caspase-12.

  • Apoptosis
  • degeneration process
  • endoplasmic reticulum stress
  • spiral ganglion neurons
  • Abbreviations

    ABRs,
    evoked auditory brain stem responses;
    ASK1,
    apoptosis signal regulating kinase 1;
    ATF6,
    activating transcription factor 6;
    CDK1,
    cyclin dependent kinase 1;
    CHOP,
    C/EBP Homologous Protein;
    dUTP,
    2′-deoxyuridine 5′-triphosphate;
    ER,
    endoplasmic reticulum;
    ERAD,
    Endoplasmic Reticulum Associated Degradation;
    IRE1,
    inositol requirement 1;
    JNK,
    c-Jun N-terminal kinase;
    LSD,
    Least significant difference;
    PERK,
    interferon-induced double stranded RNA-activated protein kinase (PRKR) -like endoplasmic reticulum kinase;
    ROS,
    reactive oxygen species;
    SGCs,
    spiral ganglion cells;
    SGN,
    spiral ganglion neuron;
    SNHL,
    severe sensorineural hearing loss;
    SPL,
    sound pressure level;
    TEM,
    transmission electron microscopy;
    TUNEL,
    terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling;
    UPR,
    unfolded protein response.
    • © 2019 The Author(s).
    http://creativecommons.org/licenses/by/4.0/

    This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

    View Full Text
    Previous ArticleNext Article
    Back to top

    February 2019

    Volume: 39 Issue: 2

    • Table of Contents
    • Index by author

    Actions

    Email

    Thank you for your interest in spreading the word about Bioscience Reports.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    Endoplasmic reticulum stress is involved in spiral ganglion neuron apoptosis following chronic kanamycin-induced deafness
    (Your Name) has forwarded a page to you from Bioscience Reports
    (Your Name) thought you would like to see this page from the Bioscience Reports web site.
    Share
    Endoplasmic reticulum stress is involved in spiral ganglion neuron apoptosis following chronic kanamycin-induced deafness
    Yaqin Tu, Guorun Fan, Haiying Sun, Xiong Cai, Wen Kong
    Bioscience Reports Feb 2019, 39 (2) BSR20181749; DOI: 10.1042/BSR20181749
    del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Facebook logo Mendeley logo
    Citation Tools
    Endoplasmic reticulum stress is involved in spiral ganglion neuron apoptosis following chronic kanamycin-induced deafness
    Yaqin Tu, Guorun Fan, Haiying Sun, Xiong Cai, Wen Kong
    Bioscience Reports Feb 2019, 39 (2) BSR20181749; DOI: 10.1042/BSR20181749

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Print
    Alerts

    Please log in to add an alert for this article.

    Request Permissions
    Save to my folders

    View Full PDF

     Open in Utopia Docs
    • Tweet Widget
    • Facebook Like

    Jump To

    • Article
      • Abstract
      • Introduction
      • Materials and methods
      • Results
      • Discussion
      • Author Contribution
      • Funding
      • Competing Interests
      • References
    • Figures
    • Info & Metrics
    • Supplementary Data
    • PDF

    Keywords

    Apoptosis
    degeneration process
    endoplasmic reticulum stress
    spiral ganglion neurons

    Related Articles

    Cited By...

    • Portland Press Homepage
    • Publish With Us
    • Advertising
    • Technical Support
    • Bioscience Reports
    • Biochemical Journal
    • Clinical Science
    • Neuronal Signaling
    • Essays in Biochemistry
    • Emerging Topics in Life Sciences
    • Biochemical Society Transactions
    • Biochemical Society Symposia
    • Cell Signalling Biology

    Portland Press Limited
    Charles Darwin House
    12 Roger Street
    London WC1N 2JU
    Email: editorial@portlandpress.com

    The Biochemical Society